The US Department of Transportation's Federal Railroad Administration (FRA) will start accepting applications for $2.1bn in grants to continue the development of high-speed intercity passenger rail corridors.
The FRA will also make available $245m for individual construction projects within the corridor.
The funds will complement the $8bn initial investment announced last January to enhance high-speed rail network in US.
The applications and proposals for the funds will be due by 6 August 2010.
The FRA is expected to grant funding for the projects by 30 September.